논문투고-심사위원수정

대한임상미생물학회

   접수번호

  20150017

   접수일

  2015-05-12

   구분

  원저

   교신저자

  이위교        (rulurala : 최경호)

   소속

  아주대학교병원 진단검사의학과

   이메일

  weegyo@ajou.ac.kr

   제목

  Vancomycin 내성 Enterococcus faecalis의 분자생물학적 특성과 전파 양상

   Running Title

  

   영문초록

  Background : Vancomycin-resistant Enterococcus (VRE) infections are caused by E. faecium in about 90% of the cases, but are rarely caused by E. faecalis. Thus, this study attempts to investigate factors that cause a low isolation rate of vancomycin-resistant E. faecalis (VREfs). To this end, the authors study the clinical traits, resistant gene structure, genomic classification, and molecular characteristics of the virulent factor.
Method : From January 2001 through September 2011, 17 vanA-containing E. faecalis isolates were collected from hospitalized patients at Ajou University Hospital in Korea. Identification, antimicrobial susceptibility testing, PCR of van and esp genes were performed. Pulsed-field gel electrophoresis (PFGE) was used for strain typing. PCR and sequencing of the internal regions of Tn1546 was performed for structural analysis of the van gene.
Result : Of 4,235 VRE, 3918 (92.5%) were E. faecium and 95 (2.2%) were E. faecalis. In 67% of VREfs infection, an occurrence of E. faecium infection predated the E. faecalis infection. All isolates were of genotype vanA. Our isolates were divided into three types according to the distribution of IS elements integrated into Tn1546 (types I to IIb). The PFGE results showed no clonal relatedness among isolates.
Conclusion : Our study finds that VREfs infections spread through patients who have experienced vancomycin-resistant E. faecium. (VREfm) infection or go through invasive procedures. The VREfs seems to involve the horizontal transfer of Tn1546 transposon from VREfm

   Key Words

  vancomycin-resistant Enterococ, Tn1546, IS1216V, IS1542

   논문파일

1 . 표지(Title Page) A-20150017-1-00.hwp 14 Kbytes 2015-05-12
2 . 본문(Main Body) A-20150017-2-00.hwp 144 Kbytes 2015-05-12
3 . 이미지(Image File) A-20150017-3-00.pptx 151 Kbytes 2015-05-12
4 . 국문초록 A-20150017-4-00.hwp 16 Kbytes 2015-05-12
5 . 본문(Main Body) 1차 수정 A-20150017-2-01.hwp 261 Kbytes 2015-07-10
6 . 국문초록 1차 수정 A-20150017-4-01.hwp 14 Kbytes 2015-07-10
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1 박정수 mdjs0721@naver.com Y 010-6276-7736 Y 2015-06-02 2015-06-17 수정후 편집위 결정
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