논문투고-심사위원수정

대한임상미생물학회

   접수번호

  20150019

   접수일

  2015-05-29

   구분

  원저

   교신저자

  박연준        (yjpk : 박연준)

   소속

  가톨릭대학교 서울성모병원

   이메일

  yjpk@catholic.ac.kr

   제목

  반코마이신내성장알균 검출을 위한 발색배지의 비교

   Running Title

  

   영문초록

  Background
Three chromogenic media using direct inoculation were compared with enterococcosel broth with enrichment step for vancomycin resistant E. faecium/E. faecalis (VRE) surveillance.
Methods
174 rectal swab specimens for VRE surveillance were included. The specimens were transferred in enterococcosel broth (EB). An aliquot of the broth was inoculated onto Brilliance VRE, chromID VRE and VRESelect and incubated upto 48h. We examined each media and EB after 24h/48h of incubation. When appropriately colored colonies were observed, identification was confirmed by VITEK-2 system and/or VITEK MS. Vancomycin susceptibility was confirmed by disk diffusion test. The presence of resistance genes was confirmed by Anyplex™ VanR Real-time.
Results
Of the 174 rectal swab specimens, 73 VRE were isolated. For enterococcosel broth, Brilliance VRE, chromID VRE and VRESelect, the sensitivity at 24h/48h was 79.2%/91.7%, 83.3%/93.1%, 79.2%/91.4% and 79.2%/ 90.3%, respectively and the specificity at 24h/48h was 85.3%/79.4%, 97.1%/85.3%, 98.0%/95.2%, and 98.0%/95.1%, respectively. The specificity of chromogenic media at 24h/48h was significantly higher than that of enterococcosel broth. Furthermore, the specificity at 48h was significantly higher for chromID VRE/VRESelect than Brilliance VRE. The color distinction was easier with VRESelect.
Conclusion
Based on our results, use of chromID VRE/VRESelect is more reliable and convenient for screening of VRE. In addition, five vanA-positive E. gallinarum, E. avium and E. durans were also isolated and two of them (one E. avium and one E. durans) were detected only on VRESelect.

   Key Words

  Oxoid, bioMérieux, Bio-Rad, VRE

   논문파일

1 . 표지(Title Page) A-20150019-1-00.docx 14 Kbytes 2015-05-29
2 . 본문(Main Body) A-20150019-2-00.docx 68 Kbytes 2015-05-29
3 . 국문초록 A-20150019-4-00.docx 15 Kbytes 2015-05-29
4 . 본문(Main Body) 1차 수정 A-20150019-2-01.docx 73 Kbytes 2015-06-22
5 . 본문(Main Body) 2차 수정 A-20150019-2-02.docx 76 Kbytes 2015-08-19
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1 류남희 nhryoo@dsmc.or.kr Y 010-2742-3115 Y 2015-06-16 2015-06-02 수정후 재심사
1 이승옥 lsok@catholic.ac.kr Y 010-2756-0736 Y 2015-06-16 2015-06-12 수정후 편집위 결정
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2 류남희 nhryoo@dsmc.or.kr Y 010-2742-3115 Y 2015-07-07 2015-08-10 수정후 편집위 결정

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