논문투고-심사위원수정

대한임상미생물학회

   접수번호

  20180012

   접수일

  2018-05-02

   구분

  원저

   교신저자

  정혜선        (kyh3545 : 김이현)

   소속

  이화여자대학교 의과대학∙의학전문대학원 진단검사의학교실

   이메일

  sunny0521.chung@ewha.ac.kr

   제목

  인유두종 바이러스 유전형 검사법 PANA RealTyper HPV kit와 AdvanSure HPV Genoblot assay의 비교

   Running Title

  

   영문초록

  Background: For the HPV genotyping, PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed which is based on multiplex real-time PCR amplification and melting curve analysis. In this research we compared PANA RealTyper to AdvanSure HPV GenoBlot assay (LG life science, Korea; AdvanSure assay) and tried to evaluate performance of PANA RealTyper.
Method: In total, 60 cervical specimens were collected from women undergoing routine cervical cancer screening. AdvanSure assay and PANA RealTyper identify same 20 genotypes in high-risk genotypes. For low-risk types, AdvanSure assay identifies 15 genotypes and PANA RealTyper identifies 2 genotypes (6 and 11) and detects 18 genotypes.
Result: Among the total 60 specimens, 54 genotypes (40 specimens) for high-risk genotypes and 20 genotypes (18 specimens) for low-risk genotypes were detected. The agreement rates of two assays were ranged from 94.4 to 100% for high-risk genotypes. Among 9 cases which were positive in PANA RealTyper but negative in AdvanSure assay, 7 cases (genotypes 16 (n=1), 39 (n=1), 52 (n=1), 58 (n=2), 68 (n=2)) were confirmed as true positive. Among 4 cases which were negative in PANA RealTyper but positive in AdvanSure assay, 3 cases were HPV genotype 59. Among the 19 cases of low risk which were positive in AdvanSure assay, there were 2 cases of HPV 6 and 1 case of HPV 11. One case of the HPV 6 cases was positive only in Pana RealTyper.
Conclusion: PANA RealTyper was comparable with AdvanSure assay. PANA RealTyper is useful and suitable for HPV genotyping.

   Key Words

  Human papilloma virus, multiplex real-time polymerase, genotype

   논문파일

1 . 표지(Title Page) A-20180012-1-00.docx 16 Kbytes 2018-05-02
2 . 본문(Main Body) A-20180012-2-00.docx 35 Kbytes 2018-05-02
3 . 국문초록 A-20180012-4-00.docx 18 Kbytes 2018-05-02
4 . 표지(Title Page) 1차 수정 A-20180012-1-01.docx 16 Kbytes 2018-10-04
5 . 본문(Main Body) 1차 수정 A-20180012-2-01.docx 57 Kbytes 2018-10-04
6 . 국문초록 1차 수정 A-20180012-4-01.docx 18 Kbytes 2018-10-04
7 . 표지(Title Page) 2차 수정 A-20180012-1-02.docx 16 Kbytes 2018-08-31
8 . 본문(Main Body) 2차 수정 A-20180012-2-02.docx 56 Kbytes 2018-08-31
9 . 국문초록 2차 수정 A-20180012-4-02.docx 18 Kbytes 2018-08-31
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