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대한임상미생물학회

   접수번호

  20180018

   접수일

  2018-08-03

   구분

  원저

   교신저자

  신정환        (jhsmile7 : 신정환)

   소속

  인제대학교 의과대학

   이메일

  jhsmile@inje.ac.kr

   제목

  카바페넴분해효소생성 장내세균 검출을 위한 Multiplex PCR의 개발 및 평가

   Running Title

  

   영문초록

  Background: The isolation of carbapenemase-producing Enterobacteriaceae (CPE) has become increasingly common. Continuous surveillance for these organisms is essential because their infections are closely related to outbreaks of illness and cause a high mortality rate. The aim of this study was to develop and evaluate multiplex PCR as a means of detecting several important CPE genes simultaneously.
Methods: We tried to develop a multiplex PCR that would detect seven CPE genes simultaneously. The multiplex PCR was composed of seven primer sets for the detection of KPC, IMP, VIM, NDM-1, GES, OXA-23, and OXA-48. We designed differences of PCR product sizes of at least 100 bp. We evaluated the performance of this new test using 69 CPE-positive clinical isolates. Also, we confirmed the specificity to rule out false-positive reactions by using 71 carbapenem-susceptible clinical strains.
Results: A total of 69 CPE clinical isolates were correctly identified as KPC (N=14), IMP (N=13), OXA-23 (N=12), OXA-48 (N=11), VIM (N=9), GES (N=5), and NDM (N=5) positive by the multiplex PCR. All 71 carbapenem-susceptible clinical isolates, including Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa, showed negative results.
Conclusion: This multiplex PCR can detect seven CPE genes at a time and would be useful in clinical laboratories.

   Key Words

  Carbapenem, Enterobacteriaceae, CRE, CPE, Multiplex PCR

   논문파일

1 . 표지(Title Page) A-20180018-1-00.docx 21 Kbytes 2018-08-03
2 . 본문(Main Body) A-20180018-2-00.docx 216 Kbytes 2018-08-03
3 . 국문초록 A-20180018-4-00.docx 22 Kbytes 2018-08-03
4 . 본문(Main Body) 1차 수정 A-20180018-2-01.docx 220 Kbytes 2018-10-03
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1 변정현 jhbyunmd@naver.com Y 010-8985-7533 Y 2018-08-27 2018-09-03 수정후 편집위 결정
1 홍기호 kihohongmd@gmail.com Y 010-7145-4916 Y 2018-08-27 2018-08-13 수정후 재심사
1 박정수 mdjs0721@naver.com Y 010-6276-7736 Y 2018-08-27 2018-09-13 게재가
2 홍기호 kihohongmd@gmail.com Y 010-7145-4916 Y 2018-10-22 2018-10-08 게재가

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