논문투고-심사위원수정

대한임상미생물학회

   접수번호

  20180020

   접수일

  2019-01-04

   구분

  원저

   교신저자

  용동은        (deyong : 용동은)

   소속

  연세대학교 의과대학 진단검사의학교실

   이메일

  deyong@yuhs.ac

   제목

  비인두 면봉 검체에서 2종의 상품화된 다중 실시간 중합효소연쇄반응 kit의 검출능 비교

   Running Title

  

   영문초록

  Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent secondary spread of infection. The main goal of this study was to compare two multiplex real-time Polymerase Chain Reaction (PCR) assays used to detect respiratory viral pathogens in nasopharyngeal swab specimens.
Methods: Between September and October 2017, a total of 84 nasopharyngeal specimens were obtained consecutively from patients in a tertiary hospital using a flocked swab with 3 ml universal transport medium (COPAN Diagnostics, Murrieta, CA, USA). A total of 64 positive and 20 negative sample results from the LG AdvanSure RV real-time RT-PCR kit were further retested using a new AdvanSure RV-plus real-time RT-PCR kit to compare their performance.
Results: Statistical analysis of positive and negative agreement between the two different kits was evaluated between the newly introduced AdvanSure RV-plus real-time RT-PCR kit and AdvanSure RV real-time RT-PCR. The overall agreement was 96.4%, with positive agreement of 98.4% and negative agreement of 90%. The evaluated sensitivity and specificity of AdvanSure RV-plus real-time RT-PCR were 96.9% and 94.7%, respectively, with a kappa value of 0.9 (P <0.001).
Conclusion: The performances of LG AdvanSure RV real-time RT-PCR and the new “AdvanSure RV-plus real-time RT-PCR” kit showed strong overall agreement. AdvanSure RV-plus real-time RT-PCR had a better detection rate. The AdvanSure RV-plus real-time RT-PCR kit can be considered a useful tool for respiratory virus diagnosis in clinical laboratories.

   Key Words

  Virus Diseases, Diagnosis, Pneumonia, Real-Time Polymerase Chain Rea, Multiplex Polymerase Chain Rea

   논문파일

1 . 표지(Title Page) A-20180020-1-00.docx 18 Kbytes 2019-01-04
2 . 본문(Main Body) A-20180020-2-00.docx 78 Kbytes 2019-01-04
3 . 국문초록 A-20180020-4-00.docx 17 Kbytes 2019-01-04
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