논문투고-심사위원수정

대한임상미생물학회

   접수번호

  20190003

   접수일

  2019-02-25

   구분

  원저

   교신저자

  용동은        (fseraph85 : 김대원)

   소속

  Department of Laboratory Medicine, Yonsei University College of Medicine

   이메일

  deyong@yuhs.ac

   제목

  Allplex RP1, 2, 3와AdvanSure RV 다중 실시간중합연쇄반응법kit간의 호흡기 바이러스 검출능 비교

   Running Title

  

   영문초록

  Background: Respiratory viruses are a major cause of acute respiratory infections leading high morbidity and mortality. Early diagnosis with the highly sensitivity and specificity PCR technique helps to timely initiate treatment and prevent further spread of infections. This study, therefore, aimed at evaluating and comparing the performance of two commercial PCR kits: Alleplex RP1, 2, 3 (Alleplex; Seegene Inc., Korea) and AdvanSure RV real-time RT-PCR (AdvanSure RV: LG Life Sciences Inc., Korea).
Method: From January to February 2017, 82 positive respiratory samples and 38 negative respiratory samples were collected. Samples were tested by AdvanSure RV then retested by AllplexTM. The Nucleic acids were extracted and prepared according to the manufacture instructions.
Results: In total, 11 respiratory viruses were detected; the positive agreement was 97.6% while the negative agreement was 89.5%. Sensitivity and specificity of Alleplex were 95.2% and 94.4% respectively. Overall agreement kappa value between AdvanSure RV and AllplexTM was 0.88 (95% CI, 0.79–0.97), P< 0.001. Alleplex showed higher detection rate in co-infected respiratory specimens.
Conclusion: The overall performance between the Alleplex and AdvanSure RV kit comparably showed very good agreement. The Alleplex had better detection rate capacity in co-infected respiratory samples. Since, Alleplex kit showed better performance for respiratory virus diagnosis, it can be a useful tool in clinical laboratories.

   Key Words

  respiratory virus, Multiplex Polymerase Chain Rea, Real-Time Polymerase Chain Rea

   논문파일

1 . 표지(Title Page) A-20190003-1-00.docx 28 Kbytes 2019-02-25
2 . 본문(Main Body) A-20190003-2-00.docx 67 Kbytes 2019-02-25
3 . 국문초록 A-20190003-4-00.docx 28 Kbytes 2019-02-25
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